Figure 6.

Zot effect on ZO-1 protein-protein interactions. Postconfluent intestinal epithelial Caco-2 cells were treated for 90 min with 30 nM Zot (lanes 2, 4, 6) or BSA (lanes 1, 3, 5), after which they were lysed; lysates were immunoprecipitated (IP) with anti-ZO-1 antibodies, and ZO-1 immunoprecipitates were processed for occludin (lanes 1, 2), claudin-1 (lanes 3, 4), and ZO-2 (lanes 5, 6) immunoblotting (IB). To ensure equal loading and transfer, PVDF membranes were stripped and reprobed with the immunoprecipitating anti-ZO-1 antibody (IB*). Immunoblots are representative of 3 independent experiments.