Figure 2.

Anti-PECAM/SOD inhibits VCAM expression in endothelial cells activated by cytokines and LPS. A, B) Confluent human endothelial cells were treated with native SOD or catalase vs. anti-PECAM/SOD or anti-PECAM/catalase for 1 h before the addition of TNF (A), IL-1β (B), or LPS. Four hours later, VCAM expression level was assessed by Western blot analysis, with normalization per actin level in the samples. Representative images are shown. C) Summarized results of ≥3 independent experiments. Data are shown as percentage of maximal activation by cytokine or LPS alone in control cells (n≥3). *P < 0.05.