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Pulmonary localization of anti-PECAM/SOD in vivo. Anesthetized mice were injected intravenously with 150 μg of anti-PECAM/SOD or control IgG/SOD. At 1 h postinjection, lungs were harvested and fixed, and tissue cryosections were immunostained as described in Materials and Methods. Colocalization of anti-PECAM/SOD (A) and IgG/SOD (B) conjugates with endosomal marker EEA-1. Scale bars = 30 μm. Arrows on phase-contrast images indicate capillaries.
