Skip to main content
. 2011 Jan;25(1):358–369. doi: 10.1096/fj.10-171579

Figure 5.

Figure 5.

Macrophages (MPs) expressed a high level of IGF-I in injured muscle. A) Quantitative RT-PCR showed that the IGF-I mRNA expression was significantly higher in injured muscle of wild-type mice than in those of Ccr2−/− mice at d 3. B) ELISA analysis showed that the level of IGF-I was increased in injured muscle of both wild-type and Ccr2−/− mice, with the peak at d 3, at which point the IGF-I level was remarkably higher in wild-type mice than in Ccr2−/− mice. C) Quantitative RT-PCR showed that the MPs from injured muscle of wild-type mice at d 3 expressed a remarkably higher level of IGF-I mRNA than the injured muscle itself from wild-type or Ccr2−/− mice. D) Quantitative RT-PCR showed that both Ly-6C+ and Ly-6C− intramuscular MPs (IM MP) expressed IGF-I mRNA, the level being significantly higher in the Ly-6C− subset than in the Ly-6C+ subset. Although the intraperitoneal MPs (IP MP) also expressed IGF-I mRNA, the level was much lower than that in the IM MPs. n = 5–7 mice/group/experiment. Fold change refers to the comparison to wild-type uninjured (UI) muscle (A, C) and to Ly-6C+ IP MPs (D). *P < 0.05; ** P < 0.01; ***P < 0.001.