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Expression of Egr-1, Fos, and Jun mRNA levels in the liver of Ames dwarf and control mice after exposure to diquat. mRNA was measured using real-time RT-PCR. Data for Egr-1, Fos, and Jun are normalized to GAPDH values and were expressed as a ratio to levels of mRNA in control mice with vehicle treatment. GAPDH values are normalized to β-actin as a loading control. Bars represent means ± se for 4 untreated or 6 diquat-treated mice of each genotype. *P < 0.05; **P < 0.01.
