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. 2011 Jan;25(1):182–191. doi: 10.1096/fj.10-164277

Figure 2.

Figure 2.

CITED2 is required for load-induced down-regulation of MMP-1. A–E) qPCR showing the effect of IHP loads (MPa as shown, 1 Hz, 1 h) applied to articular cartilage explants (n=5 rats/group) (A, B) or to chondrocytes on CITED2 and MMP-1 mRNA expression (C, D) and CITED2 and MMP-1 protein expression (E) detected by Western blot. F–H) qPCR (F, H) and Western blots (G) showing, respectively, the effect of transfecting chondrocytes with pcDNA3.1-CITED2 or small interfering RNAs for CITED2 (si-CITED2) or scrambled RNA (si-Scramble) on MMP-1 mRNA and protein expression in response to IHP (2.5 MPa, 1 Hz, 1 h) in the absence or presence of IL-1β. Experiments were performed in triplicate. *P < 0.05 vs. control.