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Examples of TSSA I, TSSA II, and TSSA I–II recognition patterns obtained in Western blot assays (A, B, and C, respectively). GST, recombinant TSSA I, and TSSA II (lanes 1, 2, and 3, respectively) were loaded in SDS-PAGE gels and transferred to PVDF membranes to perform TSSA-WB with patients sera. Representation of molecular weight pattern is depicted at the left. GST = glutathione S-transferase; TSSA = trypomastigote small-surface antigen.
