Skip to main content
. 2011 Jan 5;84(1):166–173. doi: 10.4269/ajtmh.2011.10-0438

Figure 2.

Figure 2.

Detection of species-specific DNAs by the Anopheles species multiplex polymerase chain reaction–ligase detection reaction–fluorescent microsphere assay (PCR-LDR-FMA) for mosquitoes from Papua New Guinea. Data represent a summary of 12 individual PCR LDR-FMA detecting An. koliensis (AK), An. farauti s.s. (AF), An. hinesorum (AH), An. farauti 4 (AF4), An. koliensis (AK), and Plasmodium falciparum (Pf), P. vivax (Pv), P. malariae (Pm), P. ovale (Po), Wuchereria bancrofti (Wb), human (HBS) genomic DNAs, and negative control (no DNA). Whereas genomic DNAs were added individually into PCRs for each individual mosquito species, LDR and FMA included oligonucleotide probes representing all five Anopheles species. Numbers in parentheses next to species designations in the legend ((1), (11), (59), (68), (78)) identify Luminex FlexMap™ microspheres (Luminex Corp., Austin, TX). No DNAs indicates a blank sample to which no genomic DNAs were added.