Table 5.
Concordance assessment of molecular internal transcribed spacer 2 classification methods (RFLP vs. LDR-FMA) for Anopheles punctulatus sibling species, Papua New Guinea*
| RFLP | LDR-FMA Species ID | ||||
|---|---|---|---|---|---|
| AP | AK | AF | AH | AF4 | |
| AP | 37 | – | 1 | – | – |
| AK | – | 39 | – | – | – |
| AF | – | – | 23 | – | – |
| AH | – | – | – | 3 | – |
| AF4 | 1 | – | – | – | 13 |
*
RFLP = restriction fragment length polymorphism; LDR-FMA = ligase detection reaction–fluorescent microsphere assay; ID = identification; AP = An. punctulatus s.s.; AK = An. koliensis; AF = An. farauti s.s./An. farauti 1; AH = An. hinesorum/An. farauti 2; AF4 = An. farauti 4.