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. Author manuscript; available in PMC: 2011 Jun 1.

Published in final edited form as: J Mol Biol. 2010 Oct 13;404(3):392–402. doi: 10.1016/j.jmb.2010.09.030

(a) Michaelis-Menten analysis of dicing of the 35-bp duplex. 5 nM enzyme (Dicer, Dicer-TRBP or Dicer-TRBP_pm) was incubated with 5’ ³²P-labeled substrate at the concentrations shown. (b) Michaelis-Menten analysis of dicing of pre-let-7a. 5 nM enzyme (Dicer, Dicer-TRBP or Dicer-TRBP_pm) was incubated with 5’ ³²P labeled substrate at the concentrations shown. (c) Multiple turnover assays with the 35-bp duplex and pre-let-7a comparing the activity of Dicer-TRBP_RBD3 to that of Dicer alone. Cleavage data for the 35-bp duplex is re-plotted to the right with an expanded y-axis. The plots for each substrate are marked by substrate icons, as depicted in 1a.

(a) Michaelis-Menten analysis of dicing of the 35-bp duplex. 5 nM enzyme (Dicer, Dicer-TRBP or Dicer-TRBP_pm) was incubated with 5’ ³²P-labeled substrate at the concentrations shown. (b) Michaelis-Menten analysis of dicing of pre-let-7a. 5 nM enzyme (Dicer, Dicer-TRBP or Dicer-TRBP_pm) was incubated with 5’ ³²P labeled substrate at the concentrations shown. (c) Multiple turnover assays with the 35-bp duplex and pre-let-7a comparing the activity of Dicer-TRBP_RBD3 to that of Dicer alone. Cleavage data for the 35-bp duplex is re-plotted to the right with an expanded y-axis. The plots for each substrate are marked by substrate icons, as depicted in 1a.

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