Fig. 4.

Abnormal mitochondrial networks in degenerating egg chambers of Bcl-2 family mutants. (A) Quantification of undead egg chambers compared with normal degeneration. Number of egg chambers scored is indicated above the columns. Data are percentage of undead egg chambers. (B-D) A buffy^H37 degenerating egg chamber. (F-H) A debcl^E26 degenerating egg chamber. (J-L) A debcl^E26buffy^H37 degenerating egg chamber. All samples show nuclear morphology (DNA, OliGreen), mitochondrial morphology (anti-ATP synthase, red) and merge. Arrows indicate mitochondrial islands. Scale bars: 10 μm. (E,I,M) Quantification of mitochondrial network patterns. Data are percentage of degenerating egg chambers showing the following mitochondrial network paterns: H, healthy networks as seen in healthy wild-type egg chambers; N, normal degeneration as seen in degenerating wild-type egg chambers; A, abnormal mitochondrial networks; I, abnormal networks with mitochondrial islands; n, the number of egg chambers analyzed.