Copy number of foxP3, IL10 and TGFβ expression in CD3+ T cells from blood, lymph nodes, spleen, lung graft (left) and native lung (right) from normal dogs and recipients experiencing acute lung graft rejection. A) Copy number of foxP3 was increased in CD3+ T cells from peripheral blood, lymph nodes and left (graft) lung during graft rejection. The increased foxP3 copy number in the CD3+ T cells of the lymph nodes from a recipient was notable because it was substantially higher than any other blood or tissue sample studied. B) IL10 copy number in CD3+ T cells from the peripheral blood did not significantly change during graft rejection but significantly decreased in the lymph nodes and spleen and increased in the left (graft) lung. C) TGFβ copy number in CD3+ T cells from the peripheral blood, like IL10, did not significantly change during graft rejection but significantly decreased in the lymph nodes and spleen and increased in the left (graft) lung.