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. Author manuscript; available in PMC: 2012 Jan 15.
Published in final edited form as: Eur J Pharmacol. 2010 Oct 31;650(2-3):534–543. doi: 10.1016/j.ejphar.2010.10.054

Figure 2.

Figure 2

Downregulation of muscarinic M1-M5 receptors. CHO cells stably expressing muscarinic M1 (A), M2 (B), M3 (C), M4 (D) or M5 (E) receptors were incubated in the absence (open bars) and presence (closed bars) of carbachol (1 mM) for 24 h at 37°C. Cells were washed and then used in intact, whole cell binding assays using either [3H]NMS or [3H]QNB as described in section 2.5 “Intact, whole cell receptor binding assays”. Each bar represents the mean ± S.E.M. of three experiments conducted in triplicate.