Table 2.
Comparison of the specific [3H]NMS and [3H]QNB binding for M1-M5 receptors in CHO cells incubated in the absence and presence of carbachol (1 mM) 24 hoursa.
| Receptor Subtype/Conditionb | Specific [3H]NMS Binding (fmol/mg protein) | Specific [3H]NMS Binding Remaining after Treatment (%)c | Specific [3H]QNB Binding (fmol/mg protein) | Specific [3H]QNB Binding Remaining after Treatment (%)g |
|---|---|---|---|---|
| M1 (3) | ||||
| Untreated | 748.5 ± 109.2 | 808.1 ± 116.7 | ||
| 24 h | 103.2 ± 26.6 | 13.4 ± 1.6d | 233.0 ± 64.6 | 27.8 ± 3.6h |
| M2 (3) | ||||
| Untreated | 74.9 ± 8.3 | 109.8 ± 2.3 | ||
| 24 h | 11.8 ± 1.6 | 15.7 ± 0.6e | 64.9 ± 1.4 | 59.1 ± 0.1 |
| M3 (3) | ||||
| Untreated | 471.0 ± 45.9 | 425.6 ± 31.1 | ||
| 24 h | 172.2 ± 18.8 | 36.6 ± 1.5f | 227.2 ± 13.4 | 53.5 ± 2.0 |
| M4 (3) | ||||
| Untreated | 428.2 ± 30.3 | 364.4 ± 22.6 | ||
| 24 h | 86.0 ± 8.1 | 20.1 ± 0.9 | 164.6 ± 11.5 | 45.1 ± 0.8 |
| M5 (3) | ||||
| Untreated | 195.4 ± 12.1 | 237.1 ± 20.0 | ||
| 24 h | 47.0 ± 2.5 | 24.3 ± 2.2 | 115.9 ± 10.0 | 50.0 ± 7.6 |
Data from Figure 2.
Number of experiments are shown in parenthesis.
The specific [3H]NMS binding remaining after 24 h carbachol treatment (1 mM) differed significantly across the receptor subtypes (F (4, 10) = 39.9, p < 0.0001) as determined by one-way ANOVA.
Tukey post-hoc comparisons indicate that the [3H]NMS binding remaining in CHO M1 cells (95% CI [7.5, 20]) is significantly different from M3 (95% CI [30, 43], p < 0.001) and M5 (95% CI [15, 34], p < 0.01) receptor CHO cells.
Tukey post-hoc comparisons indicate that the [3H]NMS binding remaining in CHO M2 cells (95% CI [13, 18]) is significantly different from M3 (95% CI [30, 43], p < 0.001) and M5 (95% CI [15, 34], p < 0.05) receptor CHO cells.
Tukey post-hoc comparisons indicate that the [3H]NMS binding remaining in CHO M3 cells (95% CI [30, 43]) is significantly different from M4 (95% CI [16, 24], p < 0.001) and M5 (95% CI [15, 34], p < 0.001) receptor CHO cells.
The specific [3H]QNB binding remaining after 24 h carbachol treatment (1 mM) differed significantly across the receptor subtypes (F (4, 10) = 9.4, p < 0.01) as determined by one-way ANOVA.
Tukey post-hoc comparisons indicate that the [3H]QNB binding remaining in CHO M1 cells (95% CI [12, 43]) is significantly different from M2 (95% CI [58, 59], p < 0.01), M3 (95% CI [42, 62], p < 0.01) and M5 (95% CI [17, 82], p < 0.05) receptor CHO cells.