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. Author manuscript; available in PMC: 2010 Dec 21.
Published in final edited form as: Oncogene. 2010 Aug 9;29(42):5741–5754. doi: 10.1038/onc.2010.309

Figure 5.

Figure 5

Knockdown of N-cadherin in MUC4-overexpressing cells reduces their motility. (a) The transient knockdown of N-cadherin using three pooled siRNA oligos for 48 h in SKOV3-MUC4 cells. Western blot analysis showed a downregulation of N-cadherin in siRNA-treated SKOV3-MUC4 cells compared with scramble RNA interference (RNAi)-treated SKOV3-MUC4 cells. The phosphorylated (Ser) Akt and pERK1/2 were reduced in N-cadherin knockdown cells compared with control cells. The total form of Akt and ERK, however, remained unchanged. MMP9 expression was also reduced in the N-cadherin knockdown cells. β-actin was used as a loading control. (b) Motility assay in N-cadherin knockdown SKOV3-MUC4 cells. SKOV3-MUC4 cells were treated with N-cadherin siRNA and after 24 h were trypsinized for the motility assay as described previously. There was a significant decrease in motility in the N-cadherin siRNA-treated cells compared with the scramble RNAi-treated cells (*P = 0.004; original magnification × 100, scale bar-0.8 mm).