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. Author manuscript; available in PMC: 2010 Dec 21.
Published in final edited form as: Oncogene. 2010 Aug 9;29(42):5741–5754. doi: 10.1038/onc.2010.309

Figure 6.

Figure 6

Analysis of tumor growth and metastasis in the MUC4-transfected OC cells. (a, b) In vivo tumorigenesis assay for SKOV3-MUC4 and SKOV3-vector cells with NUDE/SCID mice following subcutaneous implantation. Measurement of tumor volumes indicated that SKOV3-MUC4 cells formed significantly larger tumors as compared with control cells. Inset shows a representative image for tumors developed in SKOV3-vector- and SKOV3-MUC4-injected mice. Tumor weight was significantly higher (**P = 0.00001) in the SKOV3-MUC4 cells compared with SKOV3-vector cells. (c) In vivo metastasis analysis for SKOV3-MUC4 and SKOV3-vector cells with NUDE/SCID mice. The SKOV3-MUC4 cells injected mice showed more ascitic fluid accumulation. The ovary was found to develop tumors in both groups of mice. The SKOV3-vector-injected mice showed fewer incidence of metastasis in distant organ sites, whereas SKOV3-MUC4-injected mice showed metastatic deposits in the peritoneal wall, small intestine, colon, stomach, liver and diaphragm. (d) Hematoxylin and eosin staining of tumors produced by i.p. injection of SKOV3-vector and SKOV3-MUC4 cells. The immunohistochemical analysis of MUC4 and N-cadherin showed increased reactivity in the SKOV3-MUC4 tumors compared with SKOV3-vector tumors (original magnification × 200, scale bar-0.8 mm).