Table II.

Biochemical and Biological Impacts of Dominant-Negative Heterozygous Mutations (H391Y, K422R)(37,38)

S. no.	Features	Wild PKM2	H391Y	K422R
1	Enzymatic activity	100%	80%	25%
2	PEP affinity	Normal	Increased by sixfold	Reduced by threefold
3	Catalytic efficiency	Normal	Increased	Reduced
4	Oligomeric state	Homotetramer	Homotetramer	Homotetramer
5	Cooperativity	Allosteric	Completely lost	Increased
6	Activation by FBP	Yes	No	Yes
7	Optimum pH	7.4	7.0	7.0
8	Thermostability	No	Yes	No
9	α Helical content	Rich	Increased	Increased
10	Structural rigidity	Absent	Present	Absent
When coexpressed with PK-WT in in vitro transfection assays
11	Cross-monomer interaction	Yes	Yes	Yes
12	Oligomeric state	Normal	Disturbed	Disturbed
13	Heterooligomerization	Yes	Yes	Yes
14	Heterodimerization	No	Yes	Yes
15	Enzymatic activity	Unaffected	Reduced	Reduced
16	PEP affinity	Unaffected	Reduced	Reduced
17	E. coli doubling time	Slightly reduced	Reduced	Reduced
18	Rate of cell division	Slightly increased	Promoted	Promoted
19	Polyploidy	Unaffected	Promoted	Promoted