Figure 4.

Structural properties of Tim23N. (A) Averaged Cα/CO secondary chemical shifts observed in Tim23N as a function of residue number. Negative (positive) secondary chemical shifts spanning several residues indicate propensity for β-structure (α-helix). (B) Steady-state heteronuclear ¹⁵N-[1H]-NOEs in Tim23N at 15°C. (C) ¹⁵N R_1ρ spin relaxation rates of backbone resonances of Tim23N (black). The experimental relaxation rates follow the bulkiness profile expected from the primary sequence (gray).²¹