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Transient nature of the Tim23N-rALDH complex. (A) Helical wheel projection of the rALDH presequence. Hydrophilic residues, hydrophobic residues, negatively charged, and positively charged residues are represented by circles, diamonds, triangles, and pentagons, respectively. (B) Helical wheel projection of Tim23N (71–84). (C) Steady-state heteronuclear 15N-[1H]-NOEs observed in Tim23N without (black) and with a 64-fold excess of the rALDH presequence (gray).
