Table III.
Comparison of Kinetic Parameters of CpmtFNR/CpmtFd Toward NADPH with Those from Other Apicomplexans, Humans, and Representative Plants
| Speciesa | Type | kcat (s−1) | Km (μM) | kcat/Km (s−1 μM−1) | pH | e− Acceptor | References |
|---|---|---|---|---|---|---|---|
| CpmtFNR | Mito | 21.71 | 24.16 | 0.90 | 6.8 | cyt c | This study |
| CpmtFNR | Mito | 0.24 | 3.66 | 0.064 | 7.4 | cyt c | This study |
| CpmtFNR | Mito | 0.60 | 9.42 | 0.064 | 8.2 | cyt c | This study |
| PfFNR | Plant | 13.0 | 5.5 | 2.4 | 7.0 | cyt c | 11 |
| PfFNR | Plant | 23 | 61 | 0.37 | 8.2 | cyt c | 11 |
| TgFNR | Plant | 115 | 1.9 | 61 | 8.2 | cyt c | 16 |
| SoFNR | Plant | 180 | 5 | 36 | 8.2 | cyt c | 16 |
| ZmFNR | Plant | 227 | 7 | 32 | 8.2 | cyt c | 16 |
| HsFNR | Mito | 11.8 | 0.8 | 14.75 | 7.2 | cyt c | 35 |
a
Species initials: Cp, Cryptosporidium parvum; Pf, Plasmodium falciparum; Tg, Toxoplasma gondii; So, Spinacia oleracea; Zm: Zea Mays (root-type FNR); Hs, homo sapiens.