Figure 3.

Contraction agonists stimulate cofilin phosphorylation in canine PA rings and cultured PASMCs. Freshly dissected PA rings and differentiated PASMCs were incubated with thrombin (Thr), norepinephrine (NE), endothelin-1 (ET1), angiotensin II (AngII), and 5-hydroxytryptamine (5-HT) for 5 minutes. Total protein was extracted and resolved by SDS-PAGE, and then transferred onto nitrocellulose. Membranes were probed simultaneously with CF and P-CF antibodies. Immunoreactive bands of P-CF were normalized to immunoreactive bands of CF (insets) and the agonist-induced changes in the P-CF/CF ratio were presented as percentage of untreated controls (Ctr, bar graphs). Mean ± SD, (∗) P < .05 compared to untreated controls, n = 4.