Figure 5.

Inhibition of protein phosphatase PP2A potentiates AngII-induced phosphorylation of CF. Canine PASMCs were incubated with microcystin LF (MCLF, 20 nM) for 1 hour prior to 5 minutes incubations with angiotensin II (AngII, 1 mM) and 5-hydroxytryptamine (5-HT, 1 mM). Total protein was resolved by SDS-PAGE and then transferred onto nitrocellulose. Membranes were probed simultaneously with CF and P-CF antibodies. Immunoreactive bands of P-CF (a) were normalized to immunoreactive bands of CF (b) and the agonist-induced changes in the P-CF/CF ratio were presented as percentage of the ratio in untreated controls (Ctr, bar graphs). Mean ± SD, (∗) P < .05 compared to untreated controls, n = 3.