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. Author manuscript; available in PMC: 2011 Dec 7.
Published in final edited form as: Curr Biol. 2010 Oct 21;20(23):2078–2089. doi: 10.1016/j.cub.2010.10.008

Figure 2.

Figure 2

HUS-1::GFP and ATL-1 localize to chromatin following DNA damage, chromosome asynapsis and/or unrepaired meiotic DSBs in hermaphrodite and male germ lines. (A) Checkpoint signaling pathway in response to unrepaired recombination intermediates and IR-induced DSBs (modified from [26]). Scale bars, 5μm. (B) Fluorescence microscopy of pachytene nuclei expressing HUS-1::GFP(opIs34); both hermaphrodite and male germ cells exhibit HUS1::GFP on chromatin under checkpoint-activating conditions. Young adult worms were examined 8h after irradiation (120 Gy). Mutant and RNAi treated worms were examined 24h post L4. (C) ATL-1 is recruited to chromatin following IR or in meiotic mutants; immunolocalization of ATL-1 in fixed pachytene nuclei of adult worms (24h post L4). Young adult worms were examined 1h after IR treatment (120 Gy). Mutant and RNAi treated worms were examined 24h post L4. Scale bars, 5μm.

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