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. Author manuscript; available in PMC: 2011 Dec 7.
Published in final edited form as: Curr Biol. 2010 Oct 21;20(23):2078–2089. doi: 10.1016/j.cub.2010.10.008

Figure 3.

Figure 3

CHK-1 is expressed and phosphorylated in hermaphrodite and male germ lines. (A) Localization of CHK-1 using antibodies directed against full-length CHK-1 (red) in pachytene nuclei of wild-type and chk-1(RNAi) hermaphrodite and male germ lines counterstained with DAPI (blue). Scale bars, 5μm. (B) Localization of CHK-1 phosphorylated on Serine 345 (PO4-CHK-1; green in merge) in fixed pachytene nuclei of young adult worms 6h after IR treatment (120 Gy). Mutant and RNAi treated worms were examined 24h post L4. Scale bars, 5μm. (C) Hermaphrodite and male worm extracts analyzed by immunoblotting using antibodies to PO4- CHK-1 (~60kD), and α-tubulin (~50kD) as loading control.

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