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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: J Vasc Surg. 2010 Sep 22;52(6):1596–1607. doi: 10.1016/j.jvs.2010.06.168

Figure 6.

Figure 6

Effect of MK2i, SB or r-HSP27 treatment on the intimal layer thickening. The white bars show the intimal thickness. Vein rings (n=4–6 for each group) were cultured in RPMI medium supplemented with L-glutamine (1%), penicillin/streptomycin (1%) and FBS (30%) at 5% CO2 and 37°C for 14 days. The rings were either untreated (C) or treated with MK2i (5 or 10 μM), SB (20 μM) or r-HSP27 (19 μM). After 14 days, the rings were fixed in formalin, sectioned at 10 μm and stained using Weigert’s resorcin-fucsin. A: control rings cultured for 14 days; B: rings treated with MK2i at 5 μM; C: rings treated with MK2i at 10 μM; D: rings treated with SB at 20 μM; E: rings treated with r-HSP27; F: intimal layer thickness, G: intimal/media layer ratio: Magnification: 40X. The white arrows in panels A–E indicate the internal elastic lamina, whereas the white bars indicate the thickness of the intimal layer.