Table 1.
Protein expression results identified through SILAC-based proteomic profiling. Human dermal keloid fibroblasts treated with TGF-β (1.25 ng/ml) and TGF-β + MK2i (10 μM) for 24 h were analyzed using SILAC-labeling and MSQuant-mediated protein quantification.
| Proteins most up-regulated with TGFβ | Log2 TGF β: control | Log2: TGFβ +MK2i: control | |
|---|---|---|---|
| Gene Name | Protein Name | ratio | ratio |
| CO1A1_HUMAN | Collagen alpha-1(I) chain precursor | 0.91 | −2.53 |
| BLVRB_HUMAN | Biliverdin reductase B | 0.81 | 0.44 |
| MYO1C_HUMAN | Myosin-Ic | 0.55 | −0.67 |
| CKAP4_HUMAN | Cytoskeleton-associated protein 4 | 0.51 | 0.35 |
| SPTA2_HUMAN | Spectrin alpha chain, brain | 0.39 | −0.08 |
| HSP71_HUMAN | Heat shock 70 kDa protein 1 | 0.38 | −0.36 |
| ANXA2_HUMAN | Annexin A2 (Lipocortin II) | 0.37 | −0.6 |
| FINC_HUMAN | Fibronectin precursor | 0.3 | −0.81 |
| LIMA1_HUMAN | LIM domain and actin-binding protein 1 | 0.29 | 2.49 |
| ARP2_HUMAN | Actin-like protein 2 | 0.25 | 0.02 |
| CH60_HUMAN | 60 kDa heat shock protein, mitochondrial precursor | 0.22 | 0.92 |
| LDHA_HUMAN | L-lactate dehydrogenase A chain | 0.21 | −0.44 |
| PEA15_HUMAN | Astrocytic phosphoprotein PEA-15 | 0.2 | −0.56 |
| EZRI_HUMAN | Ezrin | 0.19 | 0.23 |
| TPM4_HUMAN | Tropomyosin alpha-4 chain | 0.17 | −1.83 |
| MOES_HUMAN | Moesin | 0.05 | −0.02 |
| DUS3_HUMAN | Dual specificity protein phosphatase 3 | 0.01 | −0.47 |
| CO6A1_HUMAN | Collagen alpha-1(VI) chain precursor | −0.01 | −0.13 |
| ANXA4_HUMAN | Annexin A4 (Lipocortin IV) | −0.09 | −0.8 |
| RS2_HUMAN | 40S ribosomal protein S2 (S4) | −0.08 | 0.24 |
| Proteins most down-regulated with TGFβ + inhibitor | |||
| RL23_HUMAN | 60S ribosomal protein L23 | −4.32 | −2.98 |
| VDAC1_HUMAN | Voltage-dependent anion-selective channel protein 1 | −0.14 | −2.72 |
| NDKA_HUMAN | Nucleoside diphosphate kinase A | −2.15 | −2.6 |
| CO1A1_HUMAN | Collagen alpha-1(I) chain precursor | 0.91 | −2.53 |
| RL27_HUMAN | 60S ribosomal protein L27 | −2.85 | −2.47 |
| CALR_HUMAN | Calreticulin precursor | −2.02 | −2.47 |
| GSTO1_HUMAN | Glutathione transferase omega-1 | −2.42 | −2.23 |
| H4_HUMAN | Histone H4 | −0.35 | −2.2 |
| GLRX1_HUMAN | Glutaredoxin-1 | −1.93 | −2.1 |
| HSPB1_HUMAN | Heat shock 27 kDa protein | −1.51 | −2.02 |
| CALD1_HUMAN | Caldesmon | −1.58 | −1.93 |
| FKB1A_HUMAN | FK506-binding protein 1A | −1.61 | −1.94 |
| ROA2_HUMAN | Heterogeneous nuclear ribonucleoproteins A2/B1 | −1.19 | −1.87 |
| TPM4_HUMAN | Tropomyosin alpha-4 chain | 0.17 | −1.83 |
| HNRPD_HUMAN | Heterogeneous nuclear ribonucleoprotein D0 | −2.07 | −1.78 |
| GSTM3_HUMAN | Glutathione S-transferase Mu 3 | −1.54 | −1.81 |
| ATP5L_HUMAN | ATP synthase subunit g, mitochondrial | −0.95 | −1.72 |
| CRYAB_HUMAN | Alpha crystallin B chain (Heat-shock protein beta-5) | −0.93 | −1.72 |
| BASP_HUMAN | Brain acid soluble protein 1 | −1.43 | −1.68 |
| ZYX_HUMAN | Zyxin | −1.28 | −1.7 |