Cells were plated as described in Materials and Methods. (A) SKOV3 cells were treated for the indicated times with 1 µM acyl-LPA. (B) Cells were stimulated with the indicated concentrations of alkyl(alk)- or acyl- 18:1 LPA for 30 minutes. After stimulation, cells were harvested, and lysates were resolved by electrophoresis, and immunoblots of transferred proteins were probed with phospho-Akt (p-T308), total Akt, phospho-ERK 1/2 (p-T202, p-Y204), or total ERK 1/2 specific antibodies. The blots are representative of greater than ten repetitions. Blots were analyzed by densitometry and fold change is presented.