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. Author manuscript; available in PMC: 2011 Dec 15.
Published in final edited form as: Free Radic Biol Med. 2010 Oct 8;49(12):2058–2067. doi: 10.1016/j.freeradbiomed.2010.10.663

Figure 2. LPA stimulates phosphorylation of Akt and ERK in SKOV3 cells.

Figure 2

Cells were plated as described in Materials and Methods. (A) SKOV3 cells were treated for the indicated times with 1 µM acyl-LPA. (B) Cells were stimulated with the indicated concentrations of alkyl(alk)- or acyl- 18:1 LPA for 30 minutes. After stimulation, cells were harvested, and lysates were resolved by electrophoresis, and immunoblots of transferred proteins were probed with phospho-Akt (p-T308), total Akt, phospho-ERK 1/2 (p-T202, p-Y204), or total ERK 1/2 specific antibodies. The blots are representative of greater than ten repetitions. Blots were analyzed by densitometry and fold change is presented.