Skip to main content
. 2010 Oct 15;20(2):261–270. doi: 10.1093/hmg/ddq460

Figure 3.

Figure 3.

(A) Effect of fisetin and Httex1-103QP-EGFP on JNK phosphorylation. PC12/HttQ103 cells were un-induced or induced with 5 μm PA in N2 medium. Fisetin (10 μm) was added to the un-induced cells or the induced cells at the time of induction as indicated. After 24h, cell extracts were prepared and equal amounts of protein were analyzed by SDS–PAGE and immunoblotting with antibodies to phospho-JNK and total JNK1. A representative blot is shown. Similar results were obtained in three independent experiments. (B) The average phosphoprotein signal from the PA-treated samples in the blots in (A), quantified by densitometry, normalized to total JNK and then normalized to the level in the absence of fisetin, was plotted as ±SD. Asterisk (*) indicates fisetin-treated versus control, P < 0.02 (unpaired t test). (C) Effect of fisetin and Httex1-103QP-EGFP on caspase 3 activation in the PC12/HttQ103 cells. Cells were un-induced or induced with 5 μm PA in N2 medium. Fisetin was added to the un-induced cells or the induced cells at the time of induction. After 48 h, caspase 3 activity was measured by caspase 3/7 Glo kit. The results are the average ± SD of three independent experiments. Asterisk (*) indicates fisetin-treated versus control, P < 0.02 (unpaired t test).