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. 2010 Dec 2;11(Suppl 4):S6. doi: 10.1186/1471-2164-11-S4-S6

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Copyright © 2010 Chen et al; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Validation of the performance of UPS 2.0 designed probes by QPCR. The expression level of nine white spot syndrome virus (WSSV) genes, determined by either QPCR or a microarray, were normalized to the measurement at 48h post viral infection. The coefficients of linear regression of gene-to-probe pairs are >0.7. The gene profile of an example, gene E, is plotted in the insert panel. The normalized measurement by QPCR and the microarray are strongly correlated for gene E (Pearson’s correlation coefficient, 0.99) and all others in this test (data not shown).