. Author manuscript; available in PMC: 2011 Oct 12.

Published in final edited form as: Biochemistry. 2010 Sep 15;49(40):8766–8778. doi: 10.1021/bi101050h

Table 3.

Transition-state binding thermodynamics for IL-7/IL-7Rα interactions

Reaction	¹ΔG^o‡ (kcal mol⁻¹)	ΔH^o‡ (kcal mol⁻¹)	−TΔS^o‡² (kcal mol⁻¹)
First-step k₁
IL-7/IL-7Rα (EC)	13.5 ± 0.2	−15.1 ± 4.4	28.6 ± 2.4
IL-7/IL-7Rα (CHO)	9.04 ± 0.1	8.3 ± 2.4	0.75 ± 2.4
First-step k₋₁
IL-7/IL-7Rα (EC)	19.7 ± 0.1	−11.1 ± 3.1	30.8 ± 2.4
IL-7/IL-7Rα (CHO)	18.7 ± 0.1	9.0 ± 1.2	9.8 ± 1.2
Second-step k₂
IL-7/IL-7Rα (EC)	20.7 ± 0.04	2.6 ± 0.7	18.1 ± 1.2
IL-7/IL-7Rα (CHO)	21.7 ± 0.1	1.2 ± 1.0	20.5 ± 1.0
Second-step k₋₂
IL-7/IL-7Rα (EC)	21.5 ± 0.1	−1.5 ± 0.9	23.0 ± 1.0
IL-7/IL-7Rα (CHO)	20.9 ± 0.1	−2.8 ± 1.3	23.7 ± 0.9

ΔG^o‡ parameters reported for 298 K. Experiments were performed with 150 mM NaCl at pH 7.4.

Calculated using −TΔS^o‡ = ΔG^‡ − ΔH^‡.