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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Eur J Cell Biol. 2010 Aug 17;90(2-3):143–156. doi: 10.1016/j.ejcb.2010.07.006

Figure 1. The SrcY527F constitutively active mutant causes extensive morphological changes in MEF cells that can be reversed by an Src inhibitor or by siRNA specific to Src.

Figure 1

(A) Control MEF and (B) MEF-SrcY527F cells were permeabilized, fixed and stained for actin (red) and paxillin (green). (C) MEF-SrcY527F cells were transiently transfected with Src siRNA and maintained in culture for 72 h before permeabilization, fixation and staining. (D) MEF-SrcY527F cells were treated with the Src family kinase inhibitor SU6656 (5μM) for 2 h and were then permeabilized, fixed, and stained for actin and paxillin.