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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Cell Signal. 2010 Jun 4;22(10):1485–1494. doi: 10.1016/j.cellsig.2010.05.017

Figure 4. IL-13 induces mRNA expression of NOX-4 and DUOX-2 in HT-29.19A and primary cultures of intestinal epithelial cells.

Figure 4

RT-PCR and real-time PCR were performed on RNA harvested from HT-29.19A cells after treatment with PBS or 20 ng/ml IL-13 using NOX-4 (Panel A), DUOX-2 (Panel B), or GAPDH (both panels) specific primers (n=3). The lane labeled + is a positive control for NOX-4 or DUOX-2 mRNA in samples in which these genes are not expressed. C) RNA was extracted from freshly isolated IEC of control donors after IL-13 stimulation, and expression of NOX-4, NOX-1, DUOX-2, p22PHOX, and GAPDH mRNA was measured by RT-PCR (n=3).