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. 2010 Dec 21;5(12):e14393. doi: 10.1371/journal.pone.0014393

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Hellman et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Claycomb media that induced increased HA synthesis in Fb was ultracentrifuged. Pellet after ultracentrifugation was dissolved in DMEM. Fb was incubated for 24 h with pellet in DMEM and Claycomb media supernatant. Fb was also incubated with fresh, unused Claycomb media. Fb, fibroblasts.