Table 2. Number of differentially expressed genes after each filtering step.

Filtering step	Cm with oHA		Cm with nHA		Fb with Cm media		Fb with Cm media sup.
Differential	554		493		1384		501
P-value<0.05	288↑	266↓	69↑	424↓	695↑	689↓	201↑	300↓
	↓		↓		↓		↓
FDR	130		105		400		102
	75↑	55↓	4↑	101↓	213↑	187↓	21↑	81↓
	↓		↓		↓		↓
Detection	111		45		335		96
P-value<0.05	68↑	43↓	3↑	42↓	177↑	158↓	21↑	75↓
	↓		↓		↓		↓
Foldchange	65		42		333		96
>1.5, <−1.5	27↑	38↓	2↑	40↓	175↑	158↓	21↑	75↓
	↓		↓		↓		↓
Avg. sign.	63		35		333		96
>50	37↑	26↓	2↑	33↓	175↑	158↓	21↑	75↓

Multiple testing corrections using False Discovery Rate was applied. A significant up-regulation was defined as a foldchange >1.5 and a significant down-regulation was defined as foldchange <−1.5. A minimum signal intensity value of 50 was utilized. Cm, cardiomyocytes; Fb, fibroblasts; sup. supernatant after ultracentrifugation, Avg. sign., average signal; FDR, False Discovery Rate; ↑, up-regulated; ↓, down-regulated.