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. 2010 Dec 21;5(12):e14394. doi: 10.1371/journal.pone.0014394

Figure 1. Dose-dependent leishmanicidal effect of DETC in Leishmania-infected human macrophages.

Figure 1

Human monocyte-derived macrophages were infected with Leishmania amazonensis promastigotes (5∶1 ratio). (A) After infection, cells were treated with increasing concentrations of DETC and the number of intracellular amastigotes (symbols) was quantified as described in Material and Methods. Each bar represents the mean ± SEM of 100 cells counted in duplicate (One-way ANOVA, ***p = 0.0001; post test for linear trend, ***p<0.001) representative of two different donors. (B) and (C) Leishmania amazonensis-infected macrophages were treated 48 h after infection with 2 mM of DETC. (B) The number of intracellular amastigotes was quantified as described in Material and Methods. Results are expressed as number of amastigotes/100 cells. Each bar represents the mean ± SEM of 4 donors (Paired t test, *p = 0.046). (C) Intracellular survival of Leishmania amazonensis amastigotes was quantified by transformation of proliferating extracellular motile promastigotes in Schneider's medium. Each bar represents the mean ± SEM of 4 donors (Paired t test, ***p = 0.0002).