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. Author manuscript; available in PMC: 2010 Dec 21.
Published in final edited form as: Circ Res. 2008 Aug 21;103(7):743–750. doi: 10.1161/CIRCRESAHA.108.172858

Figure 6.

Figure 6

Analysis of signaling pathways in Tbx3 mutant embryos at E9.5. (A, B) In situ hybridization showing elevated levels of Fgf8 transcript accumulation in the OFT of Tbx3−/− (B) relative to Tbx3+/− (A) embryos (arrowheads). (C, D) Bmp4 transcripts are reduced in the OFT of Tbx3−/− (D) relative to Tbx3+/− (C) hearts (arrowheads). (E, F) Pea3 transcripts are slightly upregulated in the caudal pharynx of Tbx3−/− embryos (F, arrowhead). (G, H) Fluorescent immunohistochemistry showing elevated levels of nuclear phospho-ERK (green) in Isl1-positive ventral pharyngeal mesoderm (red) of Tbx3−/− (H, arrowhead) compared to Tbx3+/− embryos (G). Scale bar (G, H): 100μm.