Figure 2. Recombination of AAV genomes is restricted to Cre-expressing neurons in vivo.

AAV-Stop-GFP was injected to the hippocampus of NEX-Cre transgenic mice and GFP expression visualized in brain sections two months later. Transduced CA1 pyramidal neurons express GFP (A) and Cre recombinase (B). As expected, the merge picture (C) shows segregated subcellular expression domains of the cytosolic GFP and the nuclear Cre. Bar: 25 µm.