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. Author manuscript; available in PMC: 2011 Nov 24.
Published in final edited form as: Mol Cell. 2010 Nov 4;40(4):645–657. doi: 10.1016/j.molcel.2010.10.022

Figure 5. MMS22L and NFKBIL2 suppresses intrinsic checkpoint activation and MMS22L is required for efficient recovery from the DNA damage checkpoint.

Figure 5

(A, B) Effect of MMS22L, NFKBIL2, or FACT depletion on CHK1 or CHK2 activation. Extracts from the indicated cells were immunoblotted with the indicated antibodies, using CPT (2 µM, 1h) as a checkpoint activation control.

(C) Effect of MMS22L or NFKBIL2 depletion on cell cycle progression. G1, S, and G2/M phases were determined by flow cytometry using propidium iodide staining, with the middle panel showing flow diagrams for MMS22L depletion.

(D) HeLa cells were transfected with the indicated siRNAs and after 72 h, cells were cultured with or without CPT (2 µM 1h). CPT was removed and extracts from cells analyzed for CHK2 and phospho-CHK2 (T68) at the indicated times with tubulin as a loading control.