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Microarray analysis. Intestinal epithelial IEC-6 cells were incubated in serum-free media overnight, were then treated with 2 mM or 10 mM glutamine for 24 h, and then evaluated by microarray analysis. The mRNA expression of specificity protein 3 (Sp3) (arrow) was 3.12-fold higher in 10 mM glutamine-treated cells than in 2 mM glutamine-treated cells.
