Fig. 3.

Sp3 protein expression was silenced by siRNA. A: Sp3 mRNA expression of Sp3 was decreased by 82.9% after treatment with Sp3 siRNA. After transfection with Sp3 siRNA for 48 h, total RNA was extracted, and then quantitative real-time PCR was performed. B: cytosolic Sp3 protein, but not Sp1 protein, was decreased by 74.1% after treatment of siRNA. After transfection with Sp3 siRNA for 48 h, cytoplasmic protein fractions were extracted, and then Western blot was formed. C: nuclear Sp3 protein, but not Sp1 protein, was decreased by 75.2% after treatment of siRNA. After transfection with Sp3 siRNA for 48 h, nuclear protein fractions were extracted, and then Western blot was performed.