Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Changes in the Hedgehog signaling pathway in response to vehicle and cyclopamine in the IMGE-5 cells. Quantitative RT-PCR was performed on RNA prepared from vehicle- and cyclopamine-treated IMGE-5 cells grown on permeable membranes at 0, 6, 12, 24, 48, and 72 h. Shown is the average (Av.) fold change of Ptch1 (A), Smo (B), and Gli1 (C) mRNA expression related to vehicle-treated cells. Data shown are means ± SE from 3 individual experiments. *P < 0.05 compared with response at 0 h.
