Fig. 2.

Oleate protects L6 myotubes against palmitate-induced mitochondrial DNA (mtDNA) damage and decline in ATP level. A: break frequency per 10.8 kb fragment of mtDNA after 6 h of treatment with the indicated concentrations of palmitate, oleate, or the o/p mixture. Break frequency was determined as described in materials and methods. Intensity of the band was determined by densitometry. B: L6 myotubes were treated with the indicated concentration of palmitate, oleate, or o/p mix for 24 h, and ATP production was measured. The mean results ± SE are shown (n ≥ 3). *P < 0.05.