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. 2010 Sep 21;299(6):E976–E989. doi: 10.1152/ajpendo.00448.2010

Fig. 1.

Fig. 1.

Diet-induced obese (DIO) rats are resistant to the anorexogenic action of leptin. DIO and lean rats were weighed (A), and daily food intake was determined (B) before euthanization. C: serum was obtained for leptin. D: an independent group of animals was vehicle or leptin treated (3.5 μg/rat icv) in the afternoon, and the overnight food intake was determined. E: representative microphotographs of phospho-STAT3 (pSTAT3) staining in the arcuate nucleus (ARC) of fasted lean (left) or DIO (right) rats treated with either vehicle or leptin (3.5 μg/rat icv, 30 min). Scale bars, 50 μm. F: fold stimulation in the number of pSTAT3-positive cells/section in the ARC of lean and DIO rats. G: the hypothalamic ARC was also removed from fed lean and DIO rats, and peptides were extracted for α-MSH RIA. Values are the means ± SE. *P < 0.05 vs. lean group.