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. 2010 Sep 21;299(6):E976–E989. doi: 10.1152/ajpendo.00448.2010

Fig. 4.

Fig. 4.

DIO rats have a higher metabolic rate. A and B: DIO rats have increased energy expenditure (EE) and body temperature compared with lean rats. EE was evaluated by indirect calorimetry, and the core body temperature was determined with a thermosensitive chip implanted in the interscapular region of the rats. C and D: DIO rats have higher uncoupling protein (UCP) levels compared with lean rats. To perform this, the interscapular BAT and skeletal muscle from hindlimb were extracted in RIPA lysis buffer, and UCP1 (32 kDa), UCP3 (32 kDa), and β-actin (43 kDa) levels were assayed by Western blot; n = 12/group. Values are means ± SE. The loading control β-actin is shown for all the cases. *P < 0.05 vs. lean group. AUI, arbitrary units of intensity.