Fig. 4.

Immunofluorescent analysis of macrophage phenotype. Adipose tissue samples were immunoreacted with antibodies to identify M1 and M2 macrophages. Photomicrographs are representative of a field that included a CLS, as well as interstitial macrophages. A: bright-field overlay showing triple-immunofluorescence reactivity to antibodies recognizing a pan-monocyte/macrophage marker (CD68, green), a marker preferentially expressed on M1 macrophages (CD86, blue), and a marker for M2 macrophages (CD206, red). B, C, and D: individual reactivity to CD68, CD86, and CD206, respectively. Scale bar, 50 μm.