Fig. 7.

Blocking the PI3-kinase pathway prevents PM enrichment of P63. Type II cells (A and B) or A549 cells (C and D) were incubated without additions (None) or with SP-A (1 μg/ml) without or with LY-294002 (LY; 10 μM) for either 2 h (type II cells) or 3 h (A549 cells). The cells were harvested, and PM were isolated. Representative Western blots (A and C) are shown (5 μg total membrane protein/lane). B and D: densitometric analysis of total membrane P63 protein. Data are means ± SE, n = 4 (type II cells) or n = 3 (A549 cells). *Significantly different from both None and SP-A + LY, P < 0.05.