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. 2010 Sep 10;299(6):L724–L734. doi: 10.1152/ajplung.00233.2010

Fig. 8.

Fig. 8.

Effect of KGF administration on the actin cytoskeleton. Freshly isolated AT2 cells were plated on permeable supports in control media or media supplemented with KGF, and then cultured for 5 days, fixed, stained for F-actin with Alexa 568 phalloidin, and imaged under epifluorescence (A and B) or confocal microscopy (D). A and B: KGF-treated cells (B) demonstrated significant reorganization of the actin cytoskeleton with a marked increase in the apical perijunctional F-actin ring compared with control cells (A). Inset is higher magnification image (n = at least 4 biological replicates). Scale bars, 50 μm on ×20 image and 20 μm on inset ×40 image. C: actin depolymerization with cytochalasin D treatment decreased TER to control levels (P < 0.05) without a significant decrease in control TER (n = 4 biological replicates consisting of 4–6 technical replicates). Treatment of control and KGF-treated AT2 cells with calcium-free media resulted in near complete loss of TER. Data are expressed as means ± SE. D: the perijunctional F-actin ring in KGF-treated alveolar epithelial cells (top) was disrupted by treatment with cytochalasin D (bottom) (n = at least 4 biological replicates). Scale bars, 10 μm.