Fig. 8.

A: representative image of the conserved miR-21 binding site in the 3′-untranslated region (3′-UTR) of PPARα along with the mutation site. B: luciferase reporter analysis in HEK-293 cells using a PPARα 3′-UTR assay showed direct binding by miR-21 to the 3′-UTR-region of PPARα, suggesting that PPARα could be a direct miR-21 target. Data are shown as means ± SE (n = 3); *P < 0.05 with cells containing miR-21 and 3′-UTR vs. control containing only 3′-UTR without miRNA. C: mutation in 3′-UTR of PPARα abolished binding/interaction with miR-21. SV40, simian virus 40; Poly A, a stretch of adenine nucleotides added to the 3′ end (tail) of the luciferase construct via a process called polyadenylation; hsa, Homo sapiens; mut, mutant; NS, not significant.