Skip to main content
. 2010 Sep 1;299(6):F1258–F1269. doi: 10.1152/ajprenal.00374.2010

Fig. 1.

Fig. 1.

Flow cytometry analysis to determine the phenotype of T cells infiltrating the normal and anti-glomerular basement membrane glomerulonephritis (anti-GBM GN) kidneys on day 7. After gating with a “lymphocyte gate” (based on forward and side scattering), the cell population was double-stained with mAbs for CD45RC and either CD4 or CD8α. Expression of CD3, αβTCR, γδTCR, CD62L, CD11b/c, ED-1, and NKR-P1A was analyzed after gating for CD4brightCD45RC− (gate R1), CD4dimCD45RC− (gate R2), CD8brightCD45RC− (gate R3), CD8brightCD45RC+ (gate R4), and CD8dimCD45RC+ (gate R5). Dotte-line circle indicates the gate of the CD4brightCD45RC+ cells. Numbers added near region gates in dot plot indicate percentage of gated cells within the lymphocyte gate. Shaded histograms show staining with the indicated mAbs. Open histograms show staining with isotype-matched controls.